@prefix this: <http://purl.org/np/RAyrEYfOGrC0Vk5EymBXOi2q89gnbAQgblil2gkmIuVWA> .
@prefix sub: <http://purl.org/np/RAyrEYfOGrC0Vk5EymBXOi2q89gnbAQgblil2gkmIuVWA#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:step dc:description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase.";
    a p-plan:Step .
}

sub:provenance {
  sub:assertion pav:createdWith <http://localhost:4200>;
    prov:generatedAtTime "2021-01-23T11:51:14.609881"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/miguel.ruano> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:E_06qlNvEeuWRR_bAb5r-A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
    npx:hasSignature "f5sF2eA0D0btMhdOuaTwTHFfFpCOqozwYLg00TnOy5U7NI82LrJexVlzxz5OHGB+BAm+HYvoZTbZL+uClKlHAzZ+9EazTxInfVvjJNR0Bxya2GSnr6upVPDXvKNU1MpmSxViX1qd2XyE7cePCaF20NFoTF/8BNSHvvumoLglg+I=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-23T11:51:14.609881"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}