@prefix this: <http://purl.org/np/RAxaWPt641YlZwZnklDoPYuquVWh4cobFsnKQ9hU_z2uk> .
@prefix sub: <http://purl.org/np/RAxaWPt641YlZwZnklDoPYuquVWh4cobFsnKQ9hU_z2uk#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:Nab45d600df7945b9a0e633db031a3e53 rdfs:label "tubes " .
  sub:Nd78fa2fe1d094b1da1f3c344b2bd7bae rdfs:label "swing-out bucket." .
  sub:step dc:description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase." ;
    a p-plan:Step ;
    bs:labEquipmentUsed sub:Nab45d600df7945b9a0e633db031a3e53 , sub:Nd78fa2fe1d094b1da1f3c344b2bd7bae .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-15T06:34:51.898363"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:Hfyt4FbrEeuegUefhE8ndw , authority:OubDRlbrEeuo-K9vpYq8mg , authority:U-OAPFbrEeuj2i8drh2PKg .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "FD+KWJnBHRwg3uCUG4WdCVq0jL8IsW8Jxav0w/afpujQtGHzi4k7p0A30u7ufwocNxNVurKPOOykMFVJti5IO73nAe/w5JCdqVL9x37AWjphhl3XXEbRRI+EY11HlWr/IVBU1kNklJ194mqOeKUJggzm6HClAI13bptuVdYgr2Q=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-01-15T06:34:51.898363"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}