@prefix this: <http://purl.org/np/RAwZD0FGin9xa9RmkUREIVbiYouwzNG1R3T0mz09-UC3A> .
@prefix sub: <http://purl.org/np/RAwZD0FGin9xa9RmkUREIVbiYouwzNG1R3T0mz09-UC3A#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label "sterile Dulbecco's modified eagle medium" .
  sub:_1 rdfs:label "cells" .
  sub:_2 rdfs:label "cells" .
  sub:_3 rdfs:label "sterile PBS" .
  sub:step dc:description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." ;
    sp:hasExperimentalOutput sub:_1 ;
    bs:bioSampleUsed sub:_2 ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918> , sub:_3 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-06T11:33:52.064610"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/a/acct:iannotate@hypothes.is> ;
    prov:wasDerivedFrom authority:6kGuulA8EeuEhSfeVbkZzw , authority:fbH4IlA8EeualLMBPqUcnw , authority:oY6CzlA8Eeu5KatWGDI_xA , authority:vlQhmFA8EeuN8tPze6xKgA .
}
sub:pubInfo {
   sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "YeDzfJpxx/CfXHbOqXWY9CkkdnaBqO0wuJBcM3epvAqz05iuqbAN3+kjK8NcZwRB4ALbp1QC8MHIfB97jPRmOMoaEUqZA9pcgTRqH3t/MN3J46jTg/vExCxAUrcJq10QrPsI5zwyzH8UojKKhbqHuVlO7RAXIYs3BCn1kZUc5Go=" ;
    npx:hasSignatureTarget this: .
  this: prov:generatedAtTime "2021-01-06T11:33:52.064610"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}