@prefix this: <http://purl.org/np/RAuHdvsXATIZeBo76GvRHALJRssRDYrL8-W92fBkFh8G0> .
@prefix sub: <http://purl.org/np/RAuHdvsXATIZeBo76GvRHALJRssRDYrL8-W92fBkFh8G0#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/OBI_0002488> rdfs:label "1 ml auto pipette" .
  <http://purl.obolibrary.org/obo/UBERON_0000178> rdfs:label "blood" .
  sub:_1 rdfs:label "Ficoll Histopaque" .
  sub:step dc:description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers." ;
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/UBERON_0000178> ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0000178> ;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0002488> ;
    bs:reagentUsed sub:_1 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-06T11:37:34.890266"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/a/acct:iannotate@hypothes.is> ;
    prov:wasDerivedFrom authority:4VKeYFA6EeuR7b8fbvVkwA , authority:f1WUplA6Eeuab-d0lpoWqA , authority:rcniklA6EeuKv7e3ZSEs-A , authority:wD13pFA6EeuDLuNwn-Sa_g .
}
sub:pubInfo {
   sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "Oi6biTJlfpmt/5ccmdMdxTGmC5wwwvhbi75OfZ63mfFY87UIWG28Xxwd+ltdAaRh9uo6x8vGOkPD3/PzRn41UkD+nwBPoKEwwPhogpgESSgcVbl2WFpsP2lbzLCwa8LQsKSZx3QkdA++PXCahbsuGJpwltig1gD4/TfW9Ztfa+0=" ;
    npx:hasSignatureTarget this: .
  this: prov:generatedAtTime "2021-01-06T11:37:34.890266"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}