@prefix this: <http://purl.org/np/RAuCe28_M5LxxfNVzwQ4iEYNnlFwqoU82HSy8yj1RUwsE> .
@prefix sub: <http://purl.org/np/RAuCe28_M5LxxfNVzwQ4iEYNnlFwqoU82HSy8yj1RUwsE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "2 M NaAcetate" .
  
  sub:step dc:description "Add 1/10 vol. 2 M NaAcetate (pH 5.0), equal vol. isopropanol, pellet by spinning 10 min at 3,000 x g, 4 °C.";
    a p-plan:Step;
    bs:reagentUsed sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-26T22:32:20.930949"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:Lv5zSmBPEeu5q4_R7dB-7w, authority:R3DUGGBPEeua_99lgekN-g .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "M1ZXDyQ0mILfTfRbVlJyP5q/NEfZIs0D/ihIu9S2pxVr+edE8qw69MNZeV+78lwsPeSd+cPy1c/Sn2LmqRXCJNTHeS6MO5KVDpbciEgQCWl7S6bM/5k2772/Ds+DoiHL6C4Uc+Sn2vSCM8JfDdbzavXL9HRKEFL7i/+aoqV0/YI=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-26T22:32:20.930949"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}