@prefix this: <http://purl.org/np/RAsrjnI2ldCpUQI6OrkSTq-EFNAh3l4OEbsOlB_Pu9BQk> .
@prefix sub: <http://purl.org/np/RAsrjnI2ldCpUQI6OrkSTq-EFNAh3l4OEbsOlB_Pu9BQk#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  sub:_1 rdfs:label "imaging buffer" .
  sub:_2 rdfs:label "imaging buffer" .
  <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> dc:description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." ;
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/CHEBI_29362> ;
    bs:bioSampleUsed sub:_1 ;
    bs:reagentUsed sub:_2 .
}
sub:provenance {
   sub:assertion pav:createdWith <http://localhost:4200> ;
    prov:generatedAtTime "2020-12-01T19:30:49.823471"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is> ;
    prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw , authority:FrhgjABcEeu5B4dnXgvb_A , authority:d5JrdABbEeuatj9X3vvoXw .
}
sub:pubInfo {
   sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB" ;
    npx:hasSignature "O8s4KrFLBRuS9NZTFXSFBSUmqFB57o4vefpmickwu60ObJsqHJdE2POl3USrnWna1wSY8w69RHftJYnRT6nMYWxPH/dzr/4f6176X4WZ6Cqg9mDbHOI6BSzQdRDMa+zhwQeRXjAKU5x+XcGCZkZSpy2eX0SHg2YIssQEqngnw0U=" ;
    npx:hasSignatureTarget this: .
  this: prov:generatedAtTime "2020-12-01T19:30:49.823471"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}