@prefix this: <http://purl.org/np/RAsP_4jRWbjN5W3YD1JhwYej4DOLr2085mZrfg4vZEkgw> .
@prefix sub: <http://purl.org/np/RAsP_4jRWbjN5W3YD1JhwYej4DOLr2085mZrfg4vZEkgw#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/UBERON_0000178> rdfs:label "blood " .
  
  sub:Nc0cdb5fc75d743a4ab01a5c7c9920925 rdfs:label " RPMI " .
  
  sub:Ne08ab792565b4e84a9f9829198d4d3a9 rdfs:label "apheresis collar" .
  
  sub:step dc:description "Dilute blood collected from apheresis collar with equal volume RPMI or PBS. Mix well. ";
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0000178>;
    bs:labEquipmentUsed sub:Ne08ab792565b4e84a9f9829198d4d3a9;
    bs:reagentUsed sub:Nc0cdb5fc75d743a4ab01a5c7c9920925 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-17T08:32:11.471350"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:9AYd7FjEEeuko_O0XiIBAQ, authority:C-lnjljFEeuoZCNTJKBIyg,
      authority:IJ6qLFjFEeuVvBOdAxjJ_w, authority:OkEFOFjFEeuNGo-DO0cGFQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "fASPhe2mEsFg55F2/jcj4CL3IueFcL0IUtbNJ/LNAtb9VVrhX3cdeI9GOWo55lu+22yrb0WEad+tIdFTI0aK6vthFtzb2IKD9j5hc/lhrmATqAEcJaXZxmu7lKXsirYmYDC9L+bec6LbEhUIj8xysqalgdxIND07CeMfzX08+TU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-17T08:32:11.471350"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}