http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#Head http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#provenance http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#pubInfo http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://purl.obolibrary.org/obo/CHEBI_18153 http://www.w3.org/2000/01/rdf-schema#label ethylene carbonate http://purl.obolibrary.org/obo/CHEBI_29362 http://www.w3.org/2000/01/rdf-schema#label ethylene carbonate http://purl.obolibrary.org/obo/CHEBI_41609 http://www.w3.org/2000/01/rdf-schema#label ethylene carbonate http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#_1 http://www.w3.org/2000/01/rdf-schema#label microscopy http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step http://purl.org/dc/elements/1.1/description DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis. http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.obolibrary.org/obo/CHEBI_18153 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.obolibrary.org/obo/CHEBI_29362 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.obolibrary.org/obo/CHEBI_41609 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#_1 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.obolibrary.org/obo/CHEBI_18153 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.obolibrary.org/obo/CHEBI_29362 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed http://purl.obolibrary.org/obo/CHEBI_41609 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#provenance http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://www.w3.org/ns/prov#generatedAtTime 2020-12-02T01:55:51.860012 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/a/acct:miguel.ruano@hypothes.is http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/Rq3gcP-wEeqPmLtbnUDy2A http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/icECav-xEeqGzeOtUkOIRw http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/ok_85v-xEeqsfZ9V8yVNxg http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#pubInfo http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCTAMWqX1ukrcolIABD4FqAnwdudrcdHH1xi3wTk/xbgazi7BBg0KYaCvQmw4M92siVaHAxSI5duM6oE/B3pQNGMS3doPfYEyf+j4P2v8GcYKgysWEggtdwXzeSj5vW5RW49StqA62WwlP5IYM8C6yNTf+YbWr5PwR0RGneRX1qpwIDAQAB http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#sig http://purl.org/nanopub/x/hasSignature Z83/kviYY23UP7ngmZIsKjepVzpBR8LSwsGtlx26qxB9C6skDSHHHQMRW6g26OB0daNUXcBOZrYOt9IddPGbZDTBldULs2JUxn1lDIImTotJ9jh5d1I91XCp1KzGl8qsJWpsPsZYsbqZv9W+JU2tW8P3ydg6QvXLfGFsJtgMFDM= http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.w3.org/ns/prov#generatedAtTime 2020-12-02T01:55:51.860012 http://purl.org/np/RAn_xA_oWqd-sivfynwUnB7Yg66hYIscJCb7B3206B3KU http://www.w3.org/ns/prov#wasAttributedTo https://orcid.org/0000-0002-7241-7089