@prefix this: <http://purl.org/np/RAkZ8r3l45lZz65-gpir4GjiABuzfUwSBSFZ6Yv95Zyu8> .
@prefix sub: <http://purl.org/np/RAkZ8r3l45lZz65-gpir4GjiABuzfUwSBSFZ6Yv95Zyu8#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:step dc:description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase." ;
    a p-plan:Step .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-12T00:09:01.748879"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:E_06qlNvEeuWRR_bAb5r-A .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "RTraOLXbbJ+B16o2c+FQgpf15fU0A9tdNLmrgqXrKq8qLODuSGF0O2lJ4HtIf92A9t+qWBg+FV/pfJL2PHp8Wqy4Y/u41mGrB7kXP2tIktnYz8oBS6XmgG4250XV4sk32GQFHubExyf0pKFs7KMTxqAqUfat8oYXPgbttlFgXiM=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-01-12T00:09:01.748879"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}