@prefix this: <http://purl.org/np/RAcRzhj5qQqlKKzyeV9VMl-BjLbLdQScKiJZsUXG-u0oc> .
@prefix sub: <http://purl.org/np/RAcRzhj5qQqlKKzyeV9VMl-BjLbLdQScKiJZsUXG-u0oc#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/CHEBI_53692> rdfs:label "histopaque" .
  sub:N19b5e61d6d87420ebcb74e9b0ff682ec rdfs:label "PBMCs" .
  sub:Nb9e1be3c5f5d430db0c7074d349b4577 rdfs:label "PBMCs" .
  sub:Nd397f1e2fb6e48e19438f9932771f37a rdfs:label "medium" .
  sub:step dc:description "Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium." ;
    sp:hasExperimentalOutput sub:Nb9e1be3c5f5d430db0c7074d349b4577 ;
    a p-plan:Step ;
    bs:bioSampleUsed sub:N19b5e61d6d87420ebcb74e9b0ff682ec ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_53692> , sub:Nd397f1e2fb6e48e19438f9932771f37a .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-15T06:34:22.877222"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:9udhhlbrEeungHMTQnSNRA , authority:FkKG5lbsEeungQ9zZBkQzA , authority:O9mHLlbsEeu0jUvrFwQ_uw , authority:uOfBblbrEeusAOM2O7_2zQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "c84hGKI+DmvwkeZxTKfUvTXVCarHdShENvslcmy7s5VQrYq0mg8esv1X2r23L1aN6vy1CeA3zegR7wYLsWzQS3bmHMPbB/+GbTJ36NX4HoOcEs6Rrj4TP+EXk/TdzHLaXp/yJgh76CjOAVR2fmot7nKbijIMkWts6tXwQnKU2OI=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-01-15T06:34:22.877222"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}