@prefix this: <http://purl.org/np/RA_HOYWOsvr_vKQwKgn1CnUl6rvr--DpCaYct16M73ve0> .
@prefix sub: <http://purl.org/np/RA_HOYWOsvr_vKQwKgn1CnUl6rvr--DpCaYct16M73ve0#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  <http://purl.obolibrary.org/obo/CHEBI_35225> rdfs:label "imaging buffer" .
  <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> dc:description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." ;
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/CHEBI_29362> ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_35225> ;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/CHEBI_35225> ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_35225> .
}
sub:provenance {
   sub:assertion prov:generatedAtTime "2020-11-14T19:08:45.153144"^^xsd:dateTime ;
    prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw , authority:FrhgjABcEeu5B4dnXgvb_A , authority:d5JrdABbEeuatj9X3vvoXw .
}
sub:pubInfo {
   sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB" ;
    npx:hasSignature "XR9J11yUQseV/jTvN6LFO96jnQAJnBzzYsZZF79I4o/m2DzESrCBAcIBh7Dh40Xp5Q2aULVRkfPQ2qA6jfbuatIyUZuV/xcSk/tQ0YdinYIm4UIjW+Y4FjuBstljl14RVl2Dt0eds+i8RLYbRNfQZ6XCVdjkz3g+yGnfhCG7Du0=" ;
    npx:hasSignatureTarget this: .
  this: prov:generatedAtTime "2020-11-14T19:08:45.153144"^^xsd:dateTime ;
    prov:wasAttributedTo <acct:miguel.ruano@hypothes.is> .
}