@prefix this: <http://purl.org/np/RAZr_TynWqDIWc4IWDE5cBuNOc0SVbUiIKBPhchr6XTaM> .
@prefix sub: <http://purl.org/np/RAZr_TynWqDIWc4IWDE5cBuNOc0SVbUiIKBPhchr6XTaM#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:step dc:description "Pellet down bacilli by centrifugation at 2,000 x g for 10 min, room temperature and carefully discard supernatant." .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2020-12-15T09:39:50.019514"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:iannotate@hypothes.is>;
    prov:wasDerivedFrom authority:ws6RCj7PEeuFUp8aIn07ZQ .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
    npx:hasSignature "SJkQY95RvNo2ZfQ4SN0gErpPftsDRYiqvlBoP3nGB6uKQB38cLyNEvM10NgXeweb+MTtypn1SRCYcRVcioZBljVWYs9N++GbB64hZSeZNHrZBX5w3a70vqmBy6b7DhpRVOP09rFNhAPyKj5UifOLpvgeG7huuK9lCCRf9QhOBfo=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2020-12-15T09:39:50.019514"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}