[ {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE",
    "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ],
    "http://www.nanopub.org/nschema#hasAssertion" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#assertion"
    } ],
    "http://www.nanopub.org/nschema#hasProvenance" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#provenance"
    } ],
    "http://www.nanopub.org/nschema#hasPublicationInfo" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#pubInfo"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#Head"
}, {
  "@graph" : [ {
    "@id" : "http://purl.obolibrary.org/obo/CHEBI_29362",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "ethylene carbonate"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#_1",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "imaging buffer"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#_2",
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@value" : "imaging buffer"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#step",
    "http://purl.org/dc/elements/1.1/description" : [ {
      "@value" : "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics."
    } ],
    "http://purl.org/net/SMARTprotocol#hasExperimentalInput" : [ {
      "@id" : "http://purl.obolibrary.org/obo/CHEBI_29362"
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#_1"
    } ],
    "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#_2"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#assertion"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#assertion",
    "http://purl.org/pav/createdWith" : [ {
      "@id" : "http://localhost:4200"
    } ],
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2020-12-01T20:00:50.461220"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://hypothes.is/a/acct:miguel.ruano@hypothes.is"
    } ],
    "http://www.w3.org/ns/prov#wasDerivedFrom" : [ {
      "@id" : "https://hypothes.is/a/7Z1sugBbEeu9_wtvk1iAjw"
    }, {
      "@id" : "https://hypothes.is/a/FrhgjABcEeu5B4dnXgvb_A"
    }, {
      "@id" : "https://hypothes.is/a/d5JrdABbEeuatj9X3vvoXw"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#provenance"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE",
    "http://www.w3.org/ns/prov#generatedAtTime" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2020-12-01T20:00:50.461220"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://orcid.org/0000-0002-7241-7089"
    } ]
  }, {
    "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#sig",
    "http://purl.org/nanopub/x/hasAlgorithm" : [ {
      "@value" : "RSA"
    } ],
    "http://purl.org/nanopub/x/hasPublicKey" : [ {
      "@value" : "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB"
    } ],
    "http://purl.org/nanopub/x/hasSignature" : [ {
      "@value" : "asTaQ8JvvUqYMqJzDj24qRtR8X14IN7kf+GwKjXCE+Ch7GiI9P4utDfJ5R+ON58ZqmPDymQzhpiFnMdCmSqcOHT4zBa+sCyZISqi0lWtaQZBUCG+4O+jRmvOYmcKP8+nlqv0eWK79u2q4uNAYWSorNAFSEomLeEfSHdJ5EGEIy8="
    } ],
    "http://purl.org/nanopub/x/hasSignatureTarget" : [ {
      "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE"
    } ]
  } ],
  "@id" : "http://purl.org/np/RAUOvHQYs6qU3Bvxg33puC-wvhsV6z6ZFhANzinvs2NFE#pubInfo"
} ]