@prefix this: <http://purl.org/np/RATI3e0uNX2bjB1608P_ofCBBAim3XZ9BQ2Ldv3s-bqgU> .
@prefix sub: <http://purl.org/np/RATI3e0uNX2bjB1608P_ofCBBAim3XZ9BQ2Ldv3s-bqgU#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0001100> rdfs:label "microcentrifuge tube." .
  
  sp:MicrocentrifugeTube rdfs:label "microcentrifuge tube." .
  
  sub:_1 rdfs:label "upper aqueous layer " .
  
  sub:_2 rdfs:label "microcentrifuge " .
  
  sub:step dc:description "Spin at maximum speed in a microcentrifuge for 2 minutes, transferred the upper aqueous layer to a new 2 ml microcentrifuge tube.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0001100>, sp:MicrocentrifugeTube,
      sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-26T21:18:12.725434"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:8JwQXGBAEeuMCK_6xfQJQA, authority:Kh5EqGBBEeuNhSv0Ux3bJQ,
      authority:cMPn-mBBEeu4xEtFH6Aoig, authority:k-7e4mBBEeu4xueVoXsDPA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AC8xddbk24ZXW5HaSJ3pmygc6JzvWHdJQRrFl9J5HQSXOhL5P7V/uVj8G4Cxn7qOc626UaM+ZYSstsOXe7waX646BBCTC30FC79qamPfQHIqJ68YhqyuqwkI3h9Es4hMHCCcJkMp9MPEEIy9iAUA9ffmVyP1hd7QXpWxgH3MAxg=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-26T21:18:12.725434"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}