@prefix this: <http://purl.org/np/RANTc8YIR27SBRSvRTcgZDuUdee5Ts_GAlZKjnLcYqxt4> .
@prefix sub: <http://purl.org/np/RANTc8YIR27SBRSvRTcgZDuUdee5Ts_GAlZKjnLcYqxt4#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:N2657187041cd4fee949fd4591a0bf6be rdfs:label "white layer of cells" .
  sub:Nfc211135b25c4fa9bb0910906285379d rdfs:label "media" .
  sub:step dc:description "Collect the white layer of cells just above the plastic insert into a final volume of 20 ml of media or buffer. Limit the amount of Lymphoprep that is harvested. Mix well." ;
    a p-plan:Step ;
    bs:bioSampleUsed sub:N2657187041cd4fee949fd4591a0bf6be ;
    bs:reagentUsed sub:Nfc211135b25c4fa9bb0910906285379d .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-17T08:31:36.142199"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:G5CL5FjGEeuuqLNPexWD3Q , authority:_H2THljFEeuRi0f5aWN9iA , authority:x3aH6FjFEeuRvJcamCN0LA .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "KHMxsbAXKiPnqPP3b9la9hcFA6FLffgz84eAvjXf7gY+N9Otc9XvCyMTmugvCKsEKpqTiQj3sME42268NB9O4Q5eDXImHRoc6vRgM1SleW1adTESUQde1gmpQaLdka1nQ3Cx1rk3sbUL8e0fU9GW/8N1YKQ+kqYS4ryKtN62Qgw=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-01-17T08:31:36.142199"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}