@prefix this: <http://purl.org/np/RAJKPC5ezyYfKGJE7U6hVqoBUbiHlWnFPMIVQYb79QCIQ> .
@prefix sub: <http://purl.org/np/RAJKPC5ezyYfKGJE7U6hVqoBUbiHlWnFPMIVQYb79QCIQ#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_18153> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_41609> rdfs:label "ethylene carbonate" .
  
  sub:_1 rdfs:label "microscopy" .
  
  <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> dc:description
      "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.";
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609>, sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2020-11-24T23:51:51.563826"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is>;
    prov:wasDerivedFrom authority:Rq3gcP-wEeqPmLtbnUDy2A, authority:icECav-xEeqGzeOtUkOIRw,
      authority:ok_85v-xEeqsfZ9V8yVNxg .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCTAMWqX1ukrcolIABD4FqAnwdudrcdHH1xi3wTk/xbgazi7BBg0KYaCvQmw4M92siVaHAxSI5duM6oE/B3pQNGMS3doPfYEyf+j4P2v8GcYKgysWEggtdwXzeSj5vW5RW49StqA62WwlP5IYM8C6yNTf+YbWr5PwR0RGneRX1qpwIDAQAB";
    npx:hasSignature "DOyznDLY8Pwkikti1Br0ktVR2yijj0BEYN+UNcOLT41fQIoZLPhNMGNCkUxghHDRVSxyY2RxgFwdceKaVmKLOXqAMSKb1OmF01gPwtdgS9EteW/ulr5O2lNwDUY/Vg0qG7vD4cnVMjcuQurVCUGtSeBRds8E7lGBeCL9fkb662k=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2020-11-24T23:51:51.563826"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}