@prefix this: <http://purl.org/np/RADkBLvV3oqDIroLdSEJj-8JSTola_NKmdF3a9ES0u0Vg> .
@prefix sub: <http://purl.org/np/RADkBLvV3oqDIroLdSEJj-8JSTola_NKmdF3a9ES0u0Vg#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:Nfc442fc3d97940a7bdf95b00dd411560 rdfs:label "buffer." .
  sub:step dc:description "Aspirate media and resuspend cells in 20 mL media/buffer depending on next steps." ;
    a p-plan:Step ;
    bs:reagentUsed sub:Nfc442fc3d97940a7bdf95b00dd411560 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-01-17T08:31:06.658621"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:Lf_CLFjGEeu2XxOdJw04vA , authority:YplEhljGEeu8WKO7zDHS2Q .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "et9qHwJVSkg/auW86UqL8btl23YhAtjRL8hLdhfE2LHoNXvNPvc+jKr/tJDz6zOVc3xBx0kKE/DNR4kqeAtkqeLM39MzgQv+igD4aqliFhPlmoA9ChL4PptgClJYUdFMv7o8FP4WclU+HXIgeW/l1PvJolus93dNpMlWzcmugwI=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-01-17T08:31:06.658621"^^xsd:dateTime ;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}