@prefix this: <http://purl.org/np/RAAMPqJ_mSgAIRiLVmf8ne4jLnp0FWuBEr1jPDvNplhOw> .
@prefix sub: <http://purl.org/np/RAAMPqJ_mSgAIRiLVmf8ne4jLnp0FWuBEr1jPDvNplhOw#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_18153> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  
  <http://purl.obolibrary.org/obo/CHEBI_41609> rdfs:label "ethylene carbonate" .
  
  sub:_1 rdfs:label "microscopy" .
  
  sub:step dc:description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.";
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609>, sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_18153>, <http://purl.obolibrary.org/obo/CHEBI_29362>,
      <http://purl.obolibrary.org/obo/CHEBI_41609> .
}

sub:provenance {
  sub:assertion pav:createdWith <http://localhost:4200>;
    prov:generatedAtTime "2020-12-01T23:38:09.591732"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is>;
    prov:wasDerivedFrom authority:Rq3gcP-wEeqPmLtbnUDy2A, authority:icECav-xEeqGzeOtUkOIRw,
      authority:ok_85v-xEeqsfZ9V8yVNxg .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
    npx:hasSignature "OEST9lxy0SX6+BDMXh9+IBsGur+c5rvLGFxCwFkMDQKL5DLyhNb8jPLxlNo334T5GC0Upz4esmKfDXiPt64V3ftzmiiT7TMTSQ98XGFwq3rIL4xBubLD73INfqYI+U2FWKLuGHsHWdTfVe4eLZl4Kvi8I/VaCb7kii4GQgZ6CVA=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2020-12-01T23:38:09.591732"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}