@prefix this: <http://purl.org/np/RA9q8CAl1ShohHurmYd8qO4yZg8S5Dnyje9Yaoz2kyur8> .
@prefix sub: <http://purl.org/np/RA9q8CAl1ShohHurmYd8qO4yZg8S5Dnyje9Yaoz2kyur8#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_29362> rdfs:label "ethylene carbonate" .
  
  sub:_1 rdfs:label "imaging buffer" .
  
  sub:_2 rdfs:label "imaging buffer" .
  
  sub:step dc:description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/CHEBI_29362>;
    bs:bioSampleUsed sub:_1;
    bs:reagentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2020-12-02T01:55:30.565234"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is>;
    prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw, authority:FrhgjABcEeu5B4dnXgvb_A,
      authority:d5JrdABbEeuatj9X3vvoXw .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCTAMWqX1ukrcolIABD4FqAnwdudrcdHH1xi3wTk/xbgazi7BBg0KYaCvQmw4M92siVaHAxSI5duM6oE/B3pQNGMS3doPfYEyf+j4P2v8GcYKgysWEggtdwXzeSj5vW5RW49StqA62WwlP5IYM8C6yNTf+YbWr5PwR0RGneRX1qpwIDAQAB";
    npx:hasSignature "AOecZ1SbZOWFQj4+hHpVv8dRZ4gauvr1eILCJJqYWqKPa/yMbZb1dNKtKl0/PKY8VtguwVmtHC5PfR6tdGCdxQMku903EcgtN/aj/YKFUIK40uLMZ632OYEnUIMTEBiidsiZhLvIYf0tQXBgD92rn0UPh7+8rAk7iMNDf1Swzgw=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2020-12-02T01:55:30.565234"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-7241-7089> .
}