@prefix this: <http://purl.org/np/RA72xGy0VgdGjfa-Sj3bcKt1Li07Ll2-nVOM3TDseFSVU> .
@prefix sub: <http://purl.org/np/RA72xGy0VgdGjfa-Sj3bcKt1Li07Ll2-nVOM3TDseFSVU#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  
  <http://purl.obolibrary.org/obo/CHEBI_16236> rdfs:label "70% ethanol" .
  
  <http://purl.obolibrary.org/obo/UBERON_0036018> rdfs:label "pellet " .
  
  sub:_1 rdfs:label "DNA samples" .
  
  sub:_2 rdfs:label "microcentrifuge " .
  
  sub:step dc:description " Discard supernatant and wash the pellet by adding 1.8 ml 70% ethanol, vortex thoroughly. At this stage, DNA samples can be stored at room temperature or refrigerated.";
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0036018>, sub:_1;
    bs:labEquipmentUsed sub:_2;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218>, <http://purl.obolibrary.org/obo/CHEBI_16236> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-26T21:16:44.898784"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:9OfTqmBDEeutimeZruGKXg, authority:OhdXFmBEEeuyUPsQUajIPw,
      authority:YNl9wGBEEeu6UOuSh1WfPQ, authority:iJLlpGBEEeuwmCPxCA9Neg, authority:rg9crmBEEeu1f2MhpTvsdw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "PdDX9LBqprvX6LqpPAFkzOTMJQaZRuPQi1o8Ne31OMKOTvZmh44v2NOCHlSJPTEq4iStbrgr03JRK+zQX4aupn243+ESlSpT4VlFeGvs1NTyqRrxMsv5xvNRvZZazkAMxrSegGoFaDxDN+8gGwuTk0WCLx4LmKj2Kw1YHy1/LbU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-26T21:16:44.898784"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}