sub:assertion {
sub:_1 rdfs:label " RNA " .
sub:_2 rdfs:label " RNA " .
sub:_3 rdfs:label "pellet," .
sub:_4 rdfs:label "75% and 100% ethanol," .
sub:_5 rdfs:label "DEPC-treated CES" .
sub:_6 rdfs:label " TES " .
sub:step dc:description "Wash the pellet with 75% and 100% ethanol, respectively, air dry the pellet, and dissolve the pellet with 50-200 μl DEPC-treated CES or TES (for RT PCR or other analyses involving enzymes, DEPC-treated CE or TE without SDS should be used). The total RNA yield from senescent leaves is low, so a reduced final volume is suggested." ;
sp:hasExperimentalOutput sub:_1 ;
a p-plan:Step ;
bs:bioSampleUsed sub:_2 ,
sub:_3 ;
bs:reagentUsed sub:_4 ,
sub:_5 ,
sub:_6 .
}