@prefix this: <http://purl.org/np/RA6EVRlW77B_DeGg67PtuWqvARR6vaeqKVC34oxLGVTX8> .
@prefix sub: <http://purl.org/np/RA6EVRlW77B_DeGg67PtuWqvARR6vaeqKVC34oxLGVTX8#> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "PBMCs" .
  
  sub:_2 rdfs:label "PBMCs" .
  
  sub:_3 rdfs:label "cells" .
  
  sub:_4 rdfs:label "tubes" .
  
  sub:step dc:description "The cells in interphase need to be aspirated without delay. If the tubes are kept standing for more than 10 min, PBMCs from the interphase will get disturbed and start settling down.";
    sp:hasExperimentalOutput sub:_1;
    bs:bioSampleUsed sub:_2, sub:_3;
    bs:labEquipmentUsed sub:_4 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-06T11:35:10.875901"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/a/acct:iannotate@hypothes.is>;
    prov:wasDerivedFrom authority:ECGSkFA8EeuQq1uUSNysKA, authority:KL2Q9lA8Eeu7WFujjKvVaA,
      authority:RKpLnFA8EeuKwZ8uQi11uQ, authority:a6jigFA8EeuDdMsd4eW6bg .
}

sub:pubInfo {
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "TN9lqHd+xo9CJzjx3TrbcJaDZ7fz2M0/RZpz3WEsBOXAddYZ3PKO6l3fy3ffIlh5+1ffDT8MjSnm9turCnIUwksaljwomEHn+gGuqBOujbwsi1Bd8HBDT0y0N5HMNrf1VlcLcoyuiSsVvyUlnIHxY0gNz4VNh5UKrlEfWdosMVQ=";
    npx:hasSignatureTarget this: .
  
  this: prov:generatedAtTime "2021-01-06T11:35:10.875901"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}