@prefix this: <http://purl.org/np/RA0uZ-HXPbI8WOL0rDbnl0hlsy8wWWz1CSkgzc2AoRzZE> .
@prefix sub: <http://purl.org/np/RA0uZ-HXPbI8WOL0rDbnl0hlsy8wWWz1CSkgzc2AoRzZE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "clarified supernatant" .
  
  sub:_2 rdfs:label "2 ml microcentrifuge tube " .
  
  sub:_3 rdfs:label "MM300 Mixer Mill" .
  
  sub:step dc:description "Transfer theentire clarified supernatant to a new 2 ml microcentrifuge tube contains an equal volume of chloroform. Mix well for 3-5 minutes in the MM300 Mixer Mill at 30 Hz.";
    a p-plan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed sub:_2, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-01-26T21:17:49.943652"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:H0KDGGBCEeu-dW-kmQZkwA, authority:NrWZuGBCEeuUI5-iKtxOEA,
      authority:i9lcaGBCEeuaGHPKOkmGtg, authority:rbFq1GBBEeubYH9A0WNsTQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "c89gCyElosMiZYtEdFgJYhh7PbrZ3wz0OCl2byt3EC02NRN+cNu52XRkkngQ00K3B9LTvvFXHDbmUQZpwiR7XgsAUeA8Ia9etGRg4LVrhdDUA4/jT732oWUR5E7R5vBnMuI/ezRA4JIjc5K/htlis15DAADzjOL+YLB3+egSmOk=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-01-26T21:17:49.943652"^^xsd:dateTime;
    prov:wasAttributedTo <https://orcid.org/0000-0002-3784-0922> .
}